CP Christina and Haein

Edit 0 26 …

Experimenting the Effect of Different Types of Sugar in Cellular Respiration in the Yeast


Introduction

The purpose of this lab was to determine what types of sugar affect Yeast mixture the most. The cellular respiration is the process that releases energy from food in the presence of oxygen. In order to produce energy, it requires oxygen and a food molecule. It has three stages which include Glycolysis, Krebs cycle, and Electron transport chain. The first pathway called Glycolysis takes place in cytoplasm. This pathway captures small amount of energy and locks 90% of unused chemical energy in the chemical bonds of pyruvic acid. The second stage, which is Krebs cycle, is located in Mitochondria.It also captures little amount of energy. The most energy actually comes from the last stage,the Electron transport chain. This stage uses reactants from the previous stages. Krebs cycle and Electron transport chain require Oxygen, but Glycolysis does not. In this lab, Yeast produces bubbles, because cellular respiration produces Carbon Dioxide.

Our experimental question is: does the different type of sugar affect the growth of the Yeast production? The independent variable of this experiment are different types of sugar such as white, brown, dark brown, and splenda that we blend with the Yeast mixture.The dependent variable is the amount of growth of bubbles. We used centimeters to measure the growth of bubbles, and grams to measure the weight of Yeast and sugar. Our hypothesis was:if the brown sugar is placed with the Yeast mixture, it will produce the most amount of bubbles. Because brown sugar includes the highest calories out of all 4 sugars that we used. We thought calories affect the cellular respiration, because calorie is the unit of energy that needed to raise 1 gram of 1°C water, so it means brown sugar has a lot of energy and it can increase the amount of Carbon Dioxide.


Methodology and Procedure

    1. We used white sugar, golden brown sugar, dark brown sugar, and splenda.
1st-
1. Add 3 grams of Yeast in the test tube
2. Add 25mL of water(40 degrees celsius)
3. Add 3 grams of white sugar
4. Shake the mixture
5. Wait 15 minutes
6. Measure the growth of bubble

2nd-


1. Add 3 grams of Yeast in the test tube
2. Add 25mL of water(40 degrees celsius)
3. Add 3 grams of golden sugar
4. Shake the mixture
5. Wait 15 minutes
6. Measure the growth of bubble

3rd-


1. Add 3 grams of Yeast in the test tube
2. Add 25mL of water(40 degrees celsius)
3. Add 3 grams of dark brown sugar
4. Shake the mixture
5. Wait 15 minutes
6. Measure the growth of bubble

4th-


1. Add 3 grams of Yeast in the test tube
2. Add 25mL of water(40 degrees celsius)
3. Add 3 grams of splenda
4. Shake the mixture
5. Wait 15 minutes
6. Measure the growth of bubble

Data
1. Graph
Yeast lab graph 2.PNG
2. Chart
types of sugar
 Amount of Yeast(g)
 Amount of 40°C water(mL)
 Growth of bubble(cm)
white
 3
 25
 3.2
golden brown
 3
 25
 1
dark brown
 3
 25
 1.2
splenda
 3
 25
 7


Discussion and Analysis


    1. The discussion is based on your actual results, whether they were expected or not. Explain the significance of your results. Were they what you predicted? Why or why not? How do your results relate to the “because” portion of your hypothesis?
    2. Do NOT re-write your results.
    3. This section is where you are to EXPLAIN YOUR RESULTS and what they mean. You should develop inferences based both on your experimental observations and on your prior knowledge of the topic. Your inferences must logically follow and be supported by your results. Discuss trends observed throughout your experiment.
    4. Explain why certain data was important and decide if and how the data supported your hypothesis.
    5. Discuss any weaknesses/problems in the experimental design. Identify sources of error.
    6. Suggestions for improvement and design changes to remedy errors or weakness in the design.
    7. This section is where you state whether or not the data supported the hypothesis. Ex: The data collected did not support the hypothesis. (Do not use the word “my” in this sentence.)
    8. Talk about significant results, and explain how your data fits into the body of knowledge on the topic. Avoid indicating a definite cause-and-effect relationship.
    9. Identify limitations. There are always factors outside of your control that influence your results. It’s important to identify these factors before, during, and after the investigation.
    10. Generate new questions. A good experiment raises as many or more questions than it answers. List further questions that you have and suggestions for further research.


Works Cited

    1. It is extremely important that you cite any and all sources that you use for your experiment. This includes any graphics that you use. Refer to the MLA Citation Guide in the Writing Handbook for proper format. You may skip this section if the only materials used were instructions given by your teacher (verbal or written).
    2. text book