Zach Baker and Trevor Engleman Biology 7 2/4/14 How does Food Availability affect the rate of Respiration in Yeast Cells. Introduction
Cellular Respiration is the process in which cells take food and convert it into usable energy. How does the amount of available food affect the rate at which this process is completed? We predict that the process will go faster if more food is available for use in the process.
Methodology and Procedure
To find how the rate of Respiration in yeast cells is affected by food availability, we will change the amount of sugar we give the yeast. Our lab will include yeast, water, sugar, and oil. The amount and temperature of yeast, water, and oil we use will stay the same, but we will vary the amount of sugar we give the yeast. We will put the yeast in the test tube first, followed by the water. The water will be at approximately 35-40˚ C. Next we will add the amount of sugar that we are measuring. Finally, we will shake the test tube and add a few drops of oil. We will then measure how fast the yeast grows by measuring how much it has risen every 30 seconds. The amount of sugar we are going to use will be 2 grams, 4 grams, 5 grams, 6 grams, 7 grams, and 10 grams. We will measure the amount of growth every thirty seconds for ten minutes, and record those measurements. Data and Results
The data we collected was very different from what we had expected. The tests with less food, such as the 2 (Purple) and 4 (Blue) gram experiments, did not have enough energy to sustain respiration very long. They also took longer to start reacting and the process was much slower, however in the tests with more food available ,such as the 6 (Yellow), 7 (Orange), 10 (Red) gram experiments, also performed badly, taking longer to start and reacting slower. The fastest reaction was produced by the 5 (Green) gram experiment which was one centimeter away from overflowing at the 10 minute mark.
Discussion and Analysis
The data we collected was definitely not what we were expecting. Our prediction was that if we added more sugar, than the yeast would be able to grow more, because it has more food to take in. However, there is a point where there is too much glucose for the yeast to take in. We have concluded that the optimal ratio for cellular respiration is 2.5 grams of glucose per gram of yeast. Since we were using two grams of yeast, the optimal amount of glucose was five grams. The data somewhat supported our hypothesis, but it also showed where we were mistaken. The yeast grows more with more sugar until the amount of sugar goes over the optimal ratio, and then the amount of growth starts to decrease. One problem with the lab was that sometimes we needed more time to be able to accurately record how much the yeast was going to grow. We had to stop while the yeast was still growing. We were taught that the amount of light had an optimal rate in photosynthesis, but this shows that there is an optimal rate in amount of glucose as well, and possibly other factors. We tried to make the temperature of the water consistent, but we could not control the temperature of the room each day, and this could have affected our results. It would be interesting to be able to test enough to find the best combination of glucose, light, water, and temperature for both the water and the room.
Biology 7
2/4/14
How does Food Availability affect the rate of Respiration in Yeast Cells.
Introduction
Cellular Respiration is the process in which cells take food and convert it into usable energy. How does the amount of available food affect the rate at which this process is completed? We predict that the process will go faster if more food is available for use in the process.
Methodology and Procedure
To find how the rate of Respiration in yeast cells is affected by food availability, we will change the amount of sugar we give the yeast. Our lab will include yeast, water, sugar, and oil. The amount and temperature of yeast, water, and oil we use will stay the same, but we will vary the amount of sugar we give the yeast. We will put the yeast in the test tube first, followed by the water. The water will be at approximately 35-40˚ C. Next we will add the amount of sugar that we are measuring. Finally, we will shake the test tube and add a few drops of oil. We will then measure how fast the yeast grows by measuring how much it has risen every 30 seconds. The amount of sugar we are going to use will be 2 grams, 4 grams, 5 grams, 6 grams, 7 grams, and 10 grams. We will measure the amount of growth every thirty seconds for ten minutes, and record those measurements.
Data and Results
Discussion and Analysis
The data we collected was definitely not what we were expecting. Our prediction was that if we added more sugar, than the yeast would be able to grow more, because it has more food to take in. However, there is a point where there is too much glucose for the yeast to take in. We have concluded that the optimal ratio for cellular respiration is 2.5 grams of glucose per gram of yeast. Since we were using two grams of yeast, the optimal amount of glucose was five grams. The data somewhat supported our hypothesis, but it also showed where we were mistaken. The yeast grows more with more sugar until the amount of sugar goes over the optimal ratio, and then the amount of growth starts to decrease. One problem with the lab was that sometimes we needed more time to be able to accurately record how much the yeast was going to grow. We had to stop while the yeast was still growing. We were taught that the amount of light had an optimal rate in photosynthesis, but this shows that there is an optimal rate in amount of glucose as well, and possibly other factors. We tried to make the temperature of the water consistent, but we could not control the temperature of the room each day, and this could have affected our results. It would be interesting to be able to test enough to find the best combination of glucose, light, water, and temperature for both the water and the room.
Works Cited
Baker, Zach, and Trevor Englemen. "Cellular Respiration Lab Biology." Cellular Respiration Lab Biology. N.p., 4 Feb. 14. Web. 14 Feb. 2014. <https://drive.google.com/folderview?id=0B4EMDxePMOwNRTZNRkN4Z0xkeWs&usp=sharing>.